Fluorescence Spectroscopy

Figure 1. Steady State integrated Fluorescence System for UV/VIS/IR Detection, Top View Configuration
Fluorescence Spectroscopy System

Fluorescence Spectroscopy is a powerful analytical technique with applications in biology, biochemistry, physics, chemistry and medicine. The upgradable capacity of Sciencetech Fluorometer systems provide the customer with the opportunity to begin with a low-end options and improve the system as requirements and funds increase.

Sciencetech currently offers two Steady State Fluorometer systems, models 7336 and 7556. Model 7336 is available only as a steady-state system. Model 7556 has upgrade options that include T-configurations, infrared detectors and florescence lifetime measurements in milliseconds, microseconds and nanoseconds. The standard Sciencetech Steady State Fluorometer system includes a light source, excitation monochromator, sample chamber, emission monochromator and detector.

Model 7336 uses the Sciencetech 9030 100 mm monochromator for both the excitation and emission monochromator components. The 9030 is a compact monochromator with a single concave holographic grating. Model 7556 uses Sciencetech’s 9055, quarter meter monochromator for both the excitation and emission monochromator components. The 9055 is a high resolution instrument with a triple grating turret and automatic grating switching.

Light sources for Steady State Fluorometer systems are Xenon arc lamp systems. The 7336 and 7556 both use the SC3201 transmission/ fluorescence sample chamber and a photomultiplier detector system including photomultiplier housing with power supply and photomultiplier tube. A thermoelectric cooled PMT housing with temperature controller is also available.

Model 7556 includes a Reference UV-enhanced Silicon PIN fast photodiode for synchronization when pulsed sources are used. This component is offered as an option to the 7336. Both models include all coupling optics required for source, monochromators, sample chamber and detector. Data acquisition board and system control software are included with both systems. Other accessories and options such as optical breadboards and filter wheels are also available.

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Flourescence Spectroscopy Basic System Consists of:

  1. Xe arc lamp illumination system
  2. Triplesource coupling module with rotating mirror.
    • This couples the source to excitation monochromator. The Xe lamp is connected to one of the inputs. The other two are for attachment of flash lamps or other desired wide band sources. Quartz optics.
  3. Excitation Monochromator
    • For 7336 system:
      • Our model 9030 is a compact 100 mm monochromator of Seya-Namioka type with a single concave holographic grating. Aperture is f/3.2 and the model comes with one set of fixed width slits.
      • Concave holographic grating 1200 l/mm for spectral range 200 to 800 nm
      • Manual grating control
      • Optical limit resolution 1 nm with 1200 l/mm grating
    • For 7556 system:
      • Our model 9055 direct drive monochromator is a Czerny-Turner type with an asymmetrical optical system. The triple grating turret is controlled by a computer through a RS232 serial port. Turret minimum step angle with 400 step/rev motor, is 0.0032o in half-step mode and 0.00064o in microstep mode. Concave mirror focal lengths are 200 mm input and 250 mm output. Input aperture is f/3.5. Slits have bilaterally adjustable width with micrometer control and adjustable height. Two slits are included with each monochromator.
      • One holographic grating 2400 l/mm for spectral range 200-500 nm
      • Optical limit resolution with 2400 l/mm grating better than 1.25  nm from 200 nm to 600 nm
  4. Reference UV enhanced Silicon PIN fast photodiode for synchronization for pulsed sources.
    • This photodiode is mounted near the source inside the excitation monochromator. This component is included in the 7556 system and is an option for the 7336 system.
  5. Excitation reference/coupling unit to sample chamber/laser port module.
    • This unit includes a beam-splitter quartz plate to send signals to a reference normalization photodiode. It also includes a port accessible through a flipping mirror for laser excitation. All quartz optics.
  6. Sample chamber for fluorescence systems.
    • “T” box sample chamber for possibility of adding a transmission channel with XYZ stage for sample aligning.
    • Off-axis toroidal reflective optics.
    • Angle between excitation and emission is 90°.
  7. Emission monochromator
    • For 7336 system:
      • Please see description above for further details of this component.
      • Concave holographic grating, 1200 l/mm, for spectral range 200 to 800 nm
      • Manual and automatic grating control. Stepping motor drive with microstepping capabilities. RS232 communication with computer.
      • Minimum step size of 0.06 nm
      • Optical limit resolution 1 nm with 1200 l/mm grating
      • Reciprocal linear dispersion 8 nm/mm with 1200 l/mm grating
    • For 7556 system:
      • Please see description above for further details of this component.
      • Two holographic gratings, 1200 l/mm and 600 l/mm, for spectral range 0-1200 nm
      • Optical limit resolution with 1200 l/mm grating is better than 0.5 nm from 400 nm to 1.2 µm, and with 600 l/mm grating is better than 1 µm from 1 to 2.4 µm
  8. Dual detector housing
    • With optics for coupling to PMT and infrared detector. Fliping mirror detector selection.
  9. Photomultiplier detector system.
    • Includes R928 PMT in standard non-cooled housing with high voltage power supply.
  10. Computer system.
    • Windows compatible PC and monitor, printer, standard A/D board for data acquisition. USB communication. This component included with 7556 system. Offered as an option for the 7336 system.
  11. Software
    • Detectors; data acquisition drivers.
    • Control of the monochromators: Scanning, speed, full spectra, or point by point.
    • Excitation and emission spectra observation window.
    • Data manipulation; Normalization, add, subtract, divide, multiply spectra. Baseline correction.
    • Gaussian addition.
  12. Mounting table
    • Additional options such as extra slits and gratings, filter wheels, optical tables and motor control of monochromators are possible. Please contact Sciencetech if you have any questions about options or inquiries into customizing your system.

Flourescence Spectroscopy System Models:


Table 1. Sciencetech Fluorometer Systems
ModelDescriptionPrice (USD)
7556/u Upgradeable steady state fluorometer, as described P.O.R
7336 Basic Steady state fluorometer, as described
(not including computer system)
Basic 7116 Steady state Fluorescence system P.O.R
7556 Basic 7556 with reference channel and computer control of both monochromators P.O.R

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